Multicolumn sequential separation process
US8465649B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 17, 2012 |
| Grant date | Jun 18, 2013 |
| Priority date | — |
| Expiry date | Apr 17, 2032 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D15/3804
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A process is disclosed for separating biomolecules from an aqueous solution containing the biomolectules and impurities, having different affinities and/or interactions with a solid support. The solution is passed over a fixed bed of chromatographic resin containing at least three zones, with flow of liquid being arranged between adjacent zones and between a last and first zone. Each of several sequences includes at least an adsorption stage, a rinsing stage, or a desorption stage, with each subsequent sequence being carried out by a downstream displacement of fronts in the zones by approximately the same increment before the periodical displacement of the introduction and withdrawal points.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.