Patent · US Active

Expression system for the antibiotic-free production of polypeptides

US8465946B2 · kind B2 · utility

0Cited by

1References

13Claims

0Family size

Assignee

AB Enzymes GmbH · DE

Inventors

Céline Cadot · Chaville, FR
Tina Ploss · Weiterstadt, DE
Ruth Schwerdtfeger · Darmstadt, DE
Bruno Winter · Stuttgart, DE

Key dates

Filing date	Feb 12, 2008
Grant date	Jun 18, 2013
Priority date	—
Expiry date	Aug 8, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N9/0006
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a microbial expression system for the production of polypeptides based on the use of extrachromosomal DNA, whereby no antibiotic marker genes for the selection of the host cell but DNA sequences that encode glycerine-3-phosphate dehydrogenase are used, and, thus, the production of the desired polypeptide, e.g., xylanase, does not need the addition of antibiotics. The expression system is free from antibiotic-resistance genes. The invention further relates to a DNA sequence that encodes a polypeptide with glycerine-3-phosphate dehydrogenase activity as well as a polypeptide with glycerine-3-phosphate dehydrogenase activity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.