Integrated sample preparation systems and stabilized enzyme mixtures
US8470261B2 · kind B2 · utility
3Cited by
1References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 6, 2011 |
| Grant date | Jun 25, 2013 |
| Priority date | — |
| Expiry date | Jul 7, 2031 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0683
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme, BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.