Patent · US Active

Two stage nucleic acid amplification using an amplification oligomer

US8470535B2 · kind B2 · utility

4Cited by
7References
30Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 21, 2011
Grant dateJun 25, 2013
Priority date—
Expiry dateApr 21, 2031

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6837
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.