Protein substance having triple helix structure and manufacturing method therefor
US8470555B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 22, 2009 |
| Grant date | Jun 25, 2013 |
| Priority date | — |
| Expiry date | Mar 3, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.