Group of novel enantioselective microbial nitrile hydratases with broad substrate specificity

US8470576B2 · kind B2 · utility

Assignee

• Brain Corporation · US

Inventors

• Jürgen Eck · Bensheim, DE
• Klaus Liebeton · Zwingenberg, DE
• Lutz Fischer · Stuttgart, DE
• Sabine Lutz-Wahl · Kirchheim unter Teck, DE
• Martina Hensel · Bietigheim-Bissingen, DE
• Christian Ewert · Weingarten, DE
• Marcus Wälz · Stuttgart, DE

Key dates

• Filing date: Apr 5, 2007
• Grant date: Jun 25, 2013
• Expiry date: Jul 25, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P41/006
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.