Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof

US8476021B2 · kind B2 · utility

Assignees

• PIONEER HI-BRED INTERNATIONAL, INC. · US
• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Robert F. Cressman, Jr. · Wilmington, US
• Nancy Henderson · Austin, US
• David Hondred · Altoona, US
• Nancy J. Leysens · Johnston, US
• Zhongsen Li · Hockessin, US
• Natalie N. Weber · Hockessin, US
• Aiqui Xing · Bear, US
• Cathy Ziaoyan Zhong · Wilmington, US

Key dates

• Filing date: Mar 2, 2010
• Grant date: Jul 2, 2013
• Expiry date: Nov 28, 2030

Classification

• Technology area (CPC —): General

Abstract

Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant soybean plants are provided. Specifically, soybean plants having a 3560.4.3.5 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide are provided. The soybean plant harboring the 3560.4.3.5 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:10 and/or 11. The characterization of the genomic insertion site of the 3560.4.3.5 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean 3560.4.3.5 events are provided.