Patent · US Active

Assay for evaluating affinity and specificity of ligand-albumin binding

US8476081B2 · kind B2 · utility

2Cited by

8References

10Claims

0Family size

Assignee

Janssen Pharmaceuticals, Inc. · BE

Inventors

James Kranz · Pottstown, US
Gabrielle C. Todd · Ann Arbor, US
Matthew Todd · Spring House, US

Key dates

Filing date	Sep 20, 2010
Grant date	Jul 2, 2013
Priority date	—
Expiry date	Jul 16, 2031

Classification

Technology area (CPC G)Physics
CPC primaryG01N2333/76
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A method for identifying a ligand or compound which binds to albumin comprises the steps of contacting a reaction mixture comprising a site-specific probe and albumin in the presence and the absence of the ligand or compound and measuring either dissociation constant KD, inhibitor concentration IC50 or fluorescence displacement; whereby a change in KD, IC50 and/or fluorescence in the presence of the ligand or compound is indicative of the ligand or compound binds to albumin.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.