Transgenic clawed frog embryos and use thereof as detectors of endocrine disrupters in the environment
US8476484B2 · kind B2 · utility
0Cited by
3References
5Claims
0Family size
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Key dates
| Filing date | May 27, 2003 |
| Grant date | Jul 2, 2013 |
| Priority date | — |
| Expiry date | Jan 13, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2520/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to transgenic aquatic animals, particularly the clawed frog and the zebra fish and cells derived therefrom, characterized in comprising at least one expression cassette with a regulatory DNA sequence selected from the response elements to nuclear hormone receptors, particularly TRE, connected in a functional manner downstream of a DNA segment coding for a marker protein such as luciferase or GFP. The invention further relates to methods using the transgenic cells and animals according to the invention for the identification of endocrine disrupters in the environment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.