Method for chromogenic detection of two or more target molecules in a single sample
US8481270B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 21, 2009 |
| Grant date | Jul 9, 2013 |
| Priority date | — |
| Expiry date | Apr 11, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/583
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.