Separation matrix for viral purification

US8481298B2 · kind B2 · utility

Assignee

• GE Healthcare Bio-Science AB · US

Inventors

• Inger Andersson · Varberg, SE
• Andreas Axen · Uppsala, SE
• Peder Bergvall · Uppsala, SE
• John Clachan · Uppsala, SE
• Camilla Estmer-Nilsson · Uppsala, SE
• Lena Maria Sandberg · Uppsala, SE

Key dates

• Filing date: Sep 26, 2007
• Grant date: Jul 9, 2013
• Expiry date: Jun 25, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/16051
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention.