Microfluidic device for cell culture

US8481303B2 · kind B2 · utility

Assignee

• CORNING INCORPORATED · US

Inventors

• Ronald Allen Faris · Elmira, US
• Vasiliy Nikolaevich Goral · Riverside, US
• Miya (Yi-Cheng) Hsieh · Kaohsiung, TW
• Odessa Natalie Petzold · Elmira, US
• Po Ki Yuen · Corning, US

Key dates

• Filing date: Mar 3, 2010
• Grant date: Jul 9, 2013
• Expiry date: Jul 10, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M29/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.