Method of preparing decellularized soft tissue, graft and culture material
US8486139B2 · kind B2 · utility
2Cited by
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4Claims
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Key dates
| Filing date | Mar 7, 2008 |
| Grant date | Jul 16, 2013 |
| Priority date | — |
| Expiry date | Jan 16, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/90
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
It is intended to provide a method of preparing a decellularized soft tissue whereby swelling after an ultrahigh pressure treatment can be inhibited, etc. The above-described method of preparing a decellularized soft tissue comprises the application step wherein an ultrahigh hydrostatic pressure is applied to an isolated soft tissue in a medium to thereby disrupt cells in the soft tissue, and the removal step wherein the disrupted cells are removed. As the medium, use is made of an aqueous solution containing a water-soluble polysaccharide such as dextran.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.