Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides

US8486628B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Dirk Loeffert · Haan, DE
• Christian Korfhage · Hilden, DE
• Holger Engel · Hilden, DE

Key dates

• Filing date: Jul 30, 2010
• Grant date: Jul 16, 2013
• Expiry date: Jul 30, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6851
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is related to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample.