Method of oligonucleotide sequencing by mass spectrometry
US8486707B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 25, 2011 |
| Grant date | Jul 16, 2013 |
| Priority date | — |
| Expiry date | Aug 4, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldElectrical machinery, apparatus, energy
- WIPO sectorElectrical engineering
Abstract
A computer-implemented method for confirming the nucleotide sequence of an oligonucleotide is provided. In certain embodiments, the method comprises: a) inputting the nucleotide sequence of an oligonucleotide; b) executing an algorithm that provides the predicted molecular formulas of fragments of the oligonucleotide; c) comparing the predicted m/z values of the predicted molecular formulas to experimentally-obtained m/z values obtained by analysis of the oligonucleotide by tandem mass spectrometry to determine if the predicted masses correspond with the experimentally-obtained masses. The method may be used, for example, to confirm the identity of an oligonucleotide after it is synthesized.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.