Method and apparatus for assaying test substance in sample
US8492084B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jan 24, 2007 |
| Grant date | Jul 23, 2013 |
| Priority date | — |
| Expiry date | Jan 29, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/58
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are a method for assaying a target substance in a sample and an apparatus for the method. The method can specifically assay the target substance in the sample without using any antibody against the target substance. The assaying method includes simultaneously or successively bringing a labeled aptamer, the target substance in the sample and a solid phase into contact together, and then measuring the label of the aptamer which has not been bound on the solid phase. The labeled aptamer has a property of binding to the target substance. The solid phase carries an oligonucleotide immobilized on it in an excess amount relative to the target substance. The oligonucleotide is hybridizable with the labeled aptamer when the labeled aptamer is in a state that it is not bound to the target substance, but is not hybridizable with the labeled aptamer when the labeled aptamer is in a state that it is bound to the target substance.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.