Method for extracting a protein from milk
US8492524B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 2, 2008 |
| Grant date | Jul 23, 2013 |
| Priority date | — |
| Expiry date | Mar 12, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/745
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention relates to a method for extracting a protein from milk, having at least one hydrophobic pocket and a negative charge to the natural pH of milk, that comprises the following steps: a) skimming and delipidation of the milk; b) passing the delipidated and skimmed fraction containing the protein on a chromatographic substrate on which is grafted a ligand having both a hydrophobic characteristic and an ionic characteristic in pH conditions enabling the protein to be retained on the substrate, the pH being higher than 4.6; c) elution of the protein; d) purification of the eluted fraction by removing the milk proteins from the eluted fraction; and e) recovering the protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.