Isolation of unknown rearranged T-cell receptors from single cells

US8497071B2 · kind B2 · utility

Assignee

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Alejandro Benjamin Balazs · Berkeley, US
• Jonathan Michael Tsai · Saratoga, US
• David Baltimore · Pasadena, US

Key dates

• Filing date: Jun 28, 2010
• Grant date: Jul 30, 2013
• Expiry date: Jul 30, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/3156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods and materials for isolating and identifying T cell receptors from single cells. In some embodiments, genomic DNA from a single T cell is isolated using whole genome amplification (WGA). A series of PCR reactions is carried out to enrich the genomic template for sequences encoding the TCR alpha and beta chains, and then to isolate the sequences encoding the TCR alpha and beta chains.