Method using a nonlinear optical technique for detection of interactions involving a conformational change
US8497073B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 30, 2009 |
| Grant date | Jul 30, 2013 |
| Priority date | — |
| Expiry date | Sep 30, 2029 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A nonlinear optical technique, such as second or third harmonic or sum or difference frequency generation, is used to detect binding interactions, or the degree or extent of binding, that comprise conformational change. In one aspect of the present invention, the nonlinear optical technique detects a conformational change in a probe due to target binding. In another aspect of the invention, the nonlinear optical technique screens candidate probes by detecting a conformational change due to a probe-target interaction. In another aspect of the invention, the nonlinear optical technique screens candidate modulators of a probe-target interaction by detecting a conformational change in the presence of the modulator.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.