Patent · US Active

Topoisomerase binding probe and method of use

US8497361B2 · kind B2 · utility

0Cited by

1References

20Claims

0Family size

Assignee

SRI International · US

Inventors

Peter Madrid · San Jose, US
Bryan GLASER · San Francisco, US
Jeremiah Malerich · San Jose, US

Key dates

Filing date	Aug 22, 2011
Grant date	Jul 30, 2013
Priority date	—
Expiry date	Aug 22, 2031

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/582
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

An aminocoumarin conjugated to a fluorescent label through a secondary amine, is operative as a fluorescent polarization probe of the DNA gyrase B or topoisomerase IV E subunit. The probe is used for detecting topoisomerase inhibitor binding by fluorescence polarization, particularly in a high-through put topoisomerase inhibitor assay.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.