Nitrilases and methods for making and using them
US8501451B2 · kind B2 · utility
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32References
8Claims
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Key dates
| Filing date | Sep 21, 2011 |
| Grant date | Aug 6, 2013 |
| Priority date | — |
| Expiry date | Sep 21, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.