Homologous recombination-based DNA cloning compositions
US8501454B2 · kind B2 · utility
44Cited by
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8Claims
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Key dates
| Filing date | Jan 31, 2012 |
| Grant date | Aug 6, 2013 |
| Priority date | — |
| Expiry date | Jan 31, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.