Nucleic acid modules for expression and tagging of membrane proteins and methods of use

US8501925B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Resources · US

Inventors

• Li Lin · New Taipei, TW
• John Pang · Baltimore, US
• Wen-Chi Wei · Cockeysville, US
• Edward G. Lakatta · Bel Air, US

Key dates

• Filing date: Jan 5, 2010
• Grant date: Aug 6, 2013
• Expiry date: Oct 11, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/43
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Described herein are nucleic acid modules for cloning, expression and tagging of eukaryotic membrane proteins. The nucleic acid modules include a receptor for advanced glycation end products (RAGE) signal sequence, a nucleic acid sequence encoding a tag and a multiple cloning sequence (MCS). Any membrane protein of interest can be cloned into the MCS for expression in cells. The nucleic acid modules can encode any type of tag, such as an epitope tag or affinity tag. The nucleic acid modules disclosed herein can be used to express any type of membrane protein and are particularly suited to the expression and tagging of Type I and Type III membrane proteins.