Single cell analysis by polymerase cycling assembly
US8507205B2 · kind B2 · utility
69Cited by
19References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 4, 2011 |
| Grant date | Aug 13, 2013 |
| Priority date | — |
| Expiry date | May 4, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A90/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method of making measurements on individual cells of a population, particularly cells that have identifying nucleic acid sequences, such as lymphoid cells. In one aspect, the invention provides a method of making multiparameter measurements on individual cells of such a population by carrying out a polymerase cycling assembly (PCA) reaction to link their identifying nucleic acid sequences to other cellular nucleic acids of interest. The fusion products of such PCA reaction are then sequenced and tabulated to generate multiparameter data for cells of the population.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.