Tobacco BY2 cells containing a tomato mosaic virus vector for protein production
US8507220B2 · kind B2 · utility
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Key dates
| Filing date | Oct 1, 2004 |
| Grant date | Aug 13, 2013 |
| Priority date | — |
| Expiry date | Jan 28, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8237
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.
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