Method of producing sialylated oligosaccharides
US8507227B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 7, 2007 |
| Grant date | Aug 13, 2013 |
| Priority date | — |
| Expiry date | May 1, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/26
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for the large scale in vivo synthesis of sialylated oligosaccharides, culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as lactose, wherein said microorganism comprises heterologous genes encoding a CMP-Neu5Ac synthetase, a sialic acid synthase, a GlcNAc-6-phosphate 2 epimerase and a sialyltransferase, and wherein the endogenous genes coding for sialic acid aldolase (NanA) and for ManNac kinase (NanK) have been deleted or inactivated. The invention also relates to these micoorganisms which are capable of producing internally activated sialic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.