Method of transfecting cells with nucleic acids using acidified polyethylenimine
US8507270B2 · kind B2 · utility
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9Claims
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Key dates
| Filing date | Apr 1, 2010 |
| Grant date | Aug 13, 2013 |
| Priority date | — |
| Expiry date | Sep 7, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An object of the present invention is to achieve a method capable of increasing transfection efficiency of polyethyleneimine (PEI) and reducing toxicity thereof, to provide a PEI with high transfection efficiency, and to provide a method of introducing nucleic acids into cells comprising using the PEI.There are provided a method of introducing nucleic acids into cells comprising mixing acidified PEI with nucleic acids under acidic conditions and applying the resulting mixture to cells, a reagent for introducing nucleic acids into cells comprising an acidified PEI solution, and a reagent kit including the solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.