Method for clonal amplification
US8512956B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 9, 2011 |
| Grant date | Aug 20, 2013 |
| Priority date | — |
| Expiry date | Aug 9, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present method provides methods, libraries, and kits related to the archiving and clonal amplification of sequences related to target polynucleotide sequences. The method allows for the generation and attachment of polynucleotides with defined 3′ and 5′ ends to solid surfaces. The polynucleotides attached to the solid substrates can be stored or archived as libraries and can subsequently be retrieved for analysis, for example by clonal amplification using a single composite amplification primer comprising a DNA portion and an RNA portion. In some embodiments, nucleotides attached to solid surfaces can be used for sequencing of nucleotide sequences related to the target DNA. The methods are applicable to total RNA and/or total DNA analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.