Reagent, chaotropic agent, and reagent kit for and applications of isolating nucleic acid by use of magnetic cellulose material
US8513405B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 2, 2008 |
| Grant date | Aug 20, 2013 |
| Priority date | — |
| Expiry date | Oct 24, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1013
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Disclosed are a chaotropic agent; a reagent including a chaotropic agent and a lithium salt; a reagent kit including a chaotropic agent; a chaotropic agent, a reagent, a reagent kit, and a method for isolating a nucleic acid by use of a magnetic cellulose material; a method for binding a nucleic acid to a magnetic cellulose material; a method for isolating a nucleic acid; and a method for purifying a chromosome DNA. It is required that each of the chaotropic agents, the reagents, and the reagent kits works with at least one solid-phase, magnetic cellulose-containing carrier to isolate a nucleic acid from non-nucleic acid substances. In addition, each chaotropic agent includes an alcohol substance and a substrate solution for adjusting the alcohol substance to an appropriate concentration and thereby promoting binding of the nucleic acid in a sample to the magnetic cellulose.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.