Seakem gold agarose beads comprising islets and coated with agarose

US8518394B2 · kind B2 · utility

Assignee

• THE ROGOSIN INSTITUTE · US

Inventors

• Lawrence Gazda · Xenia, US
• Barry Smith · New York, US

Key dates

• Filing date: Sep 14, 2006
• Grant date: Aug 27, 2013
• Expiry date: Nov 25, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/76
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention describes the manufacture and use of secretory cell containing bead structures that are coated with agarose. The beads, which are preferably 4 mm-12 mm in diameter, and which preferably contain islets, are made of a particular agarose, i.eagarose which has a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a Kjeldahl nitrogen content of 0-0.04 wt %. The gels found from the agarose exhibit a gel strength of at least 1200 g/cm2 (1.0 wt % concentration), substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an EEO at 1.0 wt % agarose concentration of 0.05 or less.