Patent · US Active

Regulation of protein activity by reversible acetylation

US8518635B2 · kind B2 · utility

0Cited by
4References
19Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 11, 2007
Grant dateAug 27, 2013
Priority date—
Expiry dateAug 29, 2028

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N33/573
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

This invention discloses the first cellular acetylated substrate protein of SIRT3, Acetyl-CoA synthetase 2 (AceCS2), which is a mitochondrial matrix protein. AceCS2 is reversibly acetylated at lysine 642 (Lys642) in the active site of the enzyme. The mitochondrial sirtuin SIRT3 interacts with AceCS2 and deacetylates Lys642 both in vitro and in vivo. Deacetylation of AceCS2 by SIRT3 activates the acetyl-CoA synthetase activity of AceCS2. Thus, a mammalian sirtuin directly controls the activity of a metabolic enzyme via reversible lysine acetylation. Modulators of the acetylation status or the activity of AceCS2 are useful for the treatment of pathological conditions, such as type II diabetes, hypercholesterolemia, hyperlipidemia, and obesity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.