Cellulose hydrolase and gene thereof

US8518687B2 · kind B2 · utility

Assignees

• NATIONAL PINGTUNG UNIVERSITY OF SCIENCE & TECHNOLOGY · TW
• INSTITUTE OF NUCLEAR ENERGY RESEARCH, ATOMIC ENERGY COUNCIL, EXECUTIVE YUAN · TW

Inventors

• Yo-Chia Chen · Chunri, TW
• Jeng-Chen Liu · Chunri, TW
• Hau-Yu Wang · Tainan, TW
• Teng-Chieh Hsu · Beinan, TW
• Gia-Luen Guo · New Taipei, TW
• Wen-Song Hwang · Longqi, TW
• Jia-Baau Wang · Taipei, TW

Key dates

• Filing date: Mar 20, 2012
• Grant date: Aug 27, 2013
• Expiry date: Mar 20, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01091
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.