Compositions and methods for modification of biomolecules

US8519122B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• John C. Jewett · Tucson, US
• Carolyn R. Bertozzi · Berkeley, US
• Ellen May Sletten · Los Angeles, US
• Chelsea G. Gordon · Berkeley, US

Key dates

• Filing date: Feb 10, 2011
• Grant date: Aug 27, 2013
• Expiry date: Mar 28, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/532
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided are modified cycloalkyne compounds; and methods of use of such compounds in modifying biomolecules. Embodiments include a cycloaddition reaction that can be carried out under physiological conditions. The cycloaddition reaction involves reacting a modified cycloalkyne with an azide moiety on a target biomolecule, generating a covalently modified biomolecule. The selectivity of the reaction and its compatibility with aqueous environments provide for its application in vivo and in vitro.