On-chip hybridization coupled with ITP based purification for fast sequence specific identification

US8524061B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Paul J. Utz · Portola Valley, US
• Juan G. Santiago · Fremont, US
• Michael G. Kattah · Boston, US
• Alexandre Persat · Princeton, US

Key dates

• Filing date: Nov 29, 2011
• Grant date: Sep 3, 2013
• Expiry date: May 17, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6803
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Isotachophoresis (ITP) can be employed to simultaneously focus the target and ligand of an assay into the same ITP focus zone. The target and ligand can bind to each other in the ITP focus zone, and then the resulting bound complex can be detected (e.g., by fluorescence). The sensitivity of this approach can be greatly increased by the enhanced concentration of both target and ligand that ITP provides in the focus zone. Since ITP can be performed quickly, the resulting assay is both rapid and sensitive. Markers of bacterial urinary tract infections have been experimentally detected at clinically relevant concentrations with this approach. MicroRNA sequences have also been profiled with this approach, which is clinically relevant because MicroRNA is expected to provide useful markers for disease. In one experiment, miR-122 in human kidney and liver was detected and quantified.