Culture method for amplifying large numbers of hair follicle stem cells in vitro
US8524492B2 · kind B2 · utility
5Cited by
1References
10Claims
0Family size
Assignee
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Key dates
| Filing date | Jul 20, 2011 |
| Grant date | Sep 3, 2013 |
| Priority date | — |
| Expiry date | Jul 20, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2531/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is a method for amplifying a large numbers of hair follicle stem cells in vitro by using microspheres as carriers for cell culture and a revolving bottle as a fermentation tank for cell proliferation. The method includes digesting hair follicles to obtain hair follicle stem cells, seeding the cells on microcarriers, transferring the microcarriers to a revolving bottle, feeding liquid culture media into the revolving bottle, placing the revolving bottle into a cell incubator, growing the cells on the microcarrier and harvesting the hair follicle stem cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.