Body fluid detection method using surface enhanced Raman spectroscopy
US8525980B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jun 21, 2010 |
| Grant date | Sep 3, 2013 |
| Priority date | — |
| Expiry date | Dec 11, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/447
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention provides a body fluid detection method by using surface enhanced Raman spectroscopy. In this method, some biological macromolecules in body fluid samples could be separated with membrane electrophoresis technique firstly. Next, samples are cut off along with the substrates and touched with glacial acetic acid. Transparent colloid formed while incubating. Then add enhancing substrates and continue to incubate and stir. When solid impurities precipitated, stop incubating and stand for layering. In the end, take upper layer resulted to be tested using SERS detection method and build SERS database. This invention successfully eliminated disturbance of other complex components on the SERS detection of protein, DNA and RNA. High quality SERS spectrum obtained is beneficial to the analysis and process of SERS spectrum. Thus body fluid can be differentiated by comparing body fluid SERS spectrum belonging to the healthy people and patients.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.