Enrichment of nucleic acid targets
US8529744B2 · kind B2 · utility
32Cited by
38References
26Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 23, 2012 |
| Grant date | Sep 10, 2013 |
| Priority date | — |
| Expiry date | Aug 23, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/4038
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and apparatus providing improved fidelity and specificity when separating nucleic acids from a sample, but without need for amplification. In particular, using the disclosed methods, it is possible to isolate a variant nucleic acid (i.e., a mutation) from a non-target nucleic acid (i.e., a wild-type) when the variant is present in the original sample at a much lower concentration than the non-target, e.g., 1:10,000, without substantial loss of the variant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.