Methods of inhibiting HIV infectivity

US8535684B2 · kind B2 · utility

Assignee

• Functional Genetics, Inc. · US

Inventors

• Michael S. Kinch · Laytonsville, US
• Michael Goldblatt · McLean, US
• Wu-Bo Li · North Potomac, US
• Douty Bamba · Randallstown, US
• Shaojing Chang · Rockville, US
• Huosheng Chen · Germantown, US
• Zenbework Fesseha · Columbia, US
• Manu Kohli · Dunn Loring, US
• Hanwen Mao · Annandale, US
• Heather Thi Thu Ung-Medoff · Gaithersburg, US
• Ke Weng · Gaithersburg, US

Key dates

• Filing date: Jan 6, 2010
• Grant date: Sep 17, 2013
• Expiry date: Jul 12, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1082
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.