Patent · US Active

Fluorescent proteins, their production and use

US8536314B2 · kind B2 · utility

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0References

7Claims

0Family size

Assignee

Evocatal GmbH · DE

Inventors

Thorsten Eggert · Hattingen, DE
Sascha Hausmann · Mülheim an der Ruhr, DE
Michael Puls · Monheim am Rhein, DE

Key dates

Filing date	May 19, 2010
Grant date	Sep 17, 2013
Priority date	—
Expiry date	Sep 9, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/32
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.