Promoter inducible by reactive oxygen species and vector comprising the same
US8541566B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 25, 2008 |
| Grant date | Sep 24, 2013 |
| Priority date | — |
| Expiry date | Mar 14, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/15
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides promoters inducible by reactive oxygen species (ROS), capable of driving the expression of a gene of interest, particularly in a tumor cell. More particularly, it refers to promoters inducible by reactive oxygen species, that may be used to drive the expression of a gene of interest, such as a therapeutic gene, or a reporter gene for use in image diagnosis. The promoters comprise at least a fragment of a promoter sequence responsive to said reactive oxygen species, and corresponding to a gene highly expressed in cancer cells, wherein the fragment of the promoter sequence responsive to reactive oxygen species (ROS) is selected from the group of: the VE element of the VEGF promoter, the E6 element of the promoter of the EGR-1 gene, the MMP-1 element and a chimeric promoter containing an E6 element and a VE element. It also provides vectors carrying a human therapeutic or non therapeutic gene of interest, operably linked to said promoter sequence and compositions comprising the same.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.