Reusable PCR amplification system and method

US8545769B2 · kind B2 · utility

Assignee

• The Curators of the University of Missouri · US

Inventors

• Venumadhav Korampally · Columbia, US
• Shubhra Gangopadhyay · Lubbock, US
• Keshab Gangopadhyay · Columbia, US
• Sheila Grant · Columbia, US
• Steven B. Kleiboeker · Lee's Summit, US
• Shantanu Bhattacharya · Columbia, US
• Yuanfang Gao · Columbia, US

Key dates

• Filing date: Mar 9, 2012
• Grant date: Oct 1, 2013
• Expiry date: Mar 9, 2032

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0481
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A DNA amplification device utilizing a polydimethylsiloxane (PDMS) and silicon substrate coated with spin-on glass (SOG) is provided. This PDMS layer is irreversibly bonded to the SOG layer of the silicon substrate using oxygen plasma. The amplification device is an inexpensive, microfluidic device, which can be utilized as a portable thermo-cycler to perform PCR amplification of DNA in the field.