Patent · US Active

Methods of intracellular conversion of single-chain proteins into their di-chain form

US8546108B2 · kind B2 · utility

5Cited by

27References

15Claims

0Family size

Assignee

Allergan, Inc. · US

Inventors

Sanjiv Ghanshani · Irvine, US
Linh Q. Le · Tustin, US
Yi Liu · Hsinchu, TW
Lance E. Steward · Irvine, US

Key dates

Filing date	Jan 24, 2011
Grant date	Oct 1, 2013
Priority date	—
Expiry date	Jan 24, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2799/026
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present specification discloses expression constructs comprising single-chain proteins comprising a di-chain loop region comprising an exogenous protease cleavage site and a protease that can cleave the exogenous protease cleavage site located within the di-chain loop, cell compositions comprising such expression construct, and intracellular methods of converting the single-chain protein into its di-chain form.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.