Recombinant hosts and methods for manufacturing polyhydroxyalkanoate
US8546125B2 · kind B2 · utility
1Cited by
1References
6Claims
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Key dates
| Filing date | Feb 21, 2011 |
| Grant date | Oct 1, 2013 |
| Priority date | — |
| Expiry date | Feb 21, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y401/01001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (Ppdc). It was found that the 4HB monomer content in P(3HB-co-4HB) is significantly enhanced to be over 20 mol %, in the range of 8.8 to 23 mol %.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.