Fast screening of clones
US8546145B2 · kind B2 · utility
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17Claims
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Assignee
Inventors
Key dates
| Filing date | May 27, 2011 |
| Grant date | Oct 1, 2013 |
| Priority date | — |
| Expiry date | May 27, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method of fast identification and isolation of cells featuring a desired phenotype. The phenotype is coupled to the amount of gas-liberating enzymes or increased growth. The cells are encapsulated into microcapsules allowing exchange of solvents through the microcapsule wall, but retaining some or all of the gas formed by gas-liberating enzymes on contact with corresponding substrates. Microcapsules containing increased amounts of gas-liberating enzymes are starting to float and can be separated. The cells are then isolated from the microcapsules according to standard procedures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.