Patent · US Active

Prokaryotic RNAi-like system and methods of use

US8546553B2 · kind B2 · utility

209Cited by

4References

10Claims

0Family size

Assignee

University of Georgia Research Foundation, Inc. · US

Inventors

Rebecca M. Terns · Athens, US
Michael P. Terns · Athens, US
Caryn R. Hale · Athens, US

Key dates

Filing date	Jul 24, 2009
Grant date	Oct 1, 2013
Priority date	—
Expiry date	Jul 24, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2310/3519
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided herein are methods for inactivating a target polynucleotide. The methods use a psiRNA having a 5′ region and a 3′ region. The 5′ region includes, but is not limited to, 5 to 10 nucleotides chosen from a repeat from a CRISPR locus immediately upstream of a spacer. The 3′ region is substantially complementary to a portion of the target polynucleotide. The methods may be practiced in a prokaryotic microbe or in vitro. Also provided are polypeptides that have endonuclease activity in the presence of a psiRNA and a target polynucleotide, and methods for using the polypeptides.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.