Recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen stress and the use thereof

US8546644B2 · kind B2 · utility

Assignees

• Institute of Hydrobiology, Chinese Academy of Sciences · CN
• Peking University · CN

Inventors

• Wei Hu · 红钢城街道, CN
• Zuoyan Zhu · Nanhu, CN
• Hong Ma · Seattle, US
• Bo Guan · Princeton, US
• Yuanlei Hu · Beijing, CN
• Zhongping Lin · Beijing, CN

Key dates

• Filing date: Sep 10, 2010
• Grant date: Oct 1, 2013
• Expiry date: Sep 30, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/85
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance.