Patent · US Active

Method for analysing amplified nucleic acids

US8551737B2 · kind B2 · utility

2Cited by

3References

17Claims

0Family size

Assignee

Siemens Aktiengesellschaft · DE

Inventors

Walter Gumbrecht · Herzogenaurach, DE
Peter Paulicka · Erlangen, DE
Manfred Stanzel · Erlangen, DE

Key dates

Filing date	May 31, 2007
Grant date	Oct 8, 2013
Priority date	—
Expiry date	Apr 21, 2029

Classification

Technology area (CPC B)Performing Operations; Transporting
CPC primaryB01L3/5027
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

An example embodiment of the present invention discloses a method for analyzing nucleic acids in a microfluidic device. The method includes the following steps: a) nucleic acids are amplified in a first chamber in the microfluidic device; b) the amplified nucleic acids are brought into contact with an additive including: i) monovalent cations and ii) an Mg2+ ion-binding agent, the additive being provided in a second chamber in the microfluidic device; and c) the amplified nucleic acids are hybridized on at least one probe oligonucleotide.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.