RNA polymerase mutant with improved functions
US8551752B2 · kind B2 · utility
6Cited by
3References
11Claims
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Key dates
| Filing date | Aug 7, 2009 |
| Grant date | Oct 8, 2013 |
| Priority date | — |
| Expiry date | Apr 15, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1247
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a T7 RNA polymerase mutant having improved thermal stability and/or specific activity in comparison with wild-type T7-like bacteriophage RNA polymerase, wherein at least one amino acid residue corresponding to at least one of the amino acid residues selected from the group at least consisting of glutamine at position 768, lysine at position 179 and valine at position 685 of the amino acid sequence that composes wild-type T7 RNA polymerase shown in SEQ ID NO: 6, is substituted with another amino acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.