Expression vectors containing a truncated epstein barr nuclear antigen 1 lacking the Gly-Gly-Ala domain for enhanced transient gene expression
US8551774B2 · kind B2 · utility
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3Claims
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Key dates
| Filing date | Mar 17, 2006 |
| Grant date | Oct 8, 2013 |
| Priority date | — |
| Expiry date | Sep 21, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2820/60
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to the unexpected discovery that nucleotide coding sequences coding for a truncated Epstein Barr Nuclear Antigen 1 (EBNA1t) protein (lacking the Gly-Gly-Ala domain), when in cells of mammalian origin, are associated with improved growth and increased transient gene expression when compared with cells expressing a complete EBNA1 coding sequence. The expression of EBNA1t also appear to be more stable over time.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.