Electroporation buffer composition and method of use
US8551780B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 2, 2009 |
| Grant date | Oct 8, 2013 |
| Priority date | — |
| Expiry date | Aug 10, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The subject invention concerns an electroporation buffer that allows for enhanced transfection efficiency and cell viability of cells during application of an electric current. Buffers of the invention provide for maximum transfer of target particles into cells while maintaining the health and growth potential of the cell population. Compositions of the invention comprise electroporation buffers of approximately physiological ionic strength and pH, and having serum or purified proteins, such as serum albumin, added thereto. The subject invention is suitable for use with any cell type. The subject invention also concerns methods of electroporation using an electroporation buffer of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.